Wednesday, January 26, 2011

Neurophysiology Lab

Purpose:
     To try and identify the neurons based on the morphology and response to stimuli, comparing them to previous results. We are measuring the voltage of individual neurons, that we assume are familiar with the concept of voltages. Which is only electrical measurement you are going to make.

Hypothesis:
     I believe that  each neuron that is delivered to the the leech will have a different reaction and morphyology to the stimuli.

Materials:
     A glass microelectrode, a micromanicpulator, an oscilloscope, a digital volmeter, leech tank, leech tongs, dissection trey, dissecting microscope, probe, forceps, scissors, scalpal, discretion pins

Procedure:
  1. Anesthetize and dissect the leech 
  2. Place the dissected tissue in a salt solution
  3. Cut out the ganglion window
  4. Isolate one ganglion
  5. Cut the ganglion sinus
  6. Probe and identify ganglion sensory cells
Results:
     Completing this lab, i have realized that the activities of neurons had the same response or morphology to the stimuli. Each neuron inside the leech had different cell types.

Conclusion:
     While doing this lab i have come to a conclusion that the individual electrical activities inside the neuron each have different reactions to the stimuli. Then having to identify the neurons and compare them came easy to me, mostly because i had a guide to help me.